An evaluation of the anticancer activity in Hopea odorata extracts Norizah, A1*, Zakiah, I2 and Wan, Zurinah WN2
1Herbal Medicine Research Centre, Institute for Medical Research, Jalan Pahang 50588 Kuala Lumpur, Malaysia. 2Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 KL, Malaysia
Abstract Possible anticancer characteristics of Hopea Odorata extracts were investigated by cell proliferation and viability studies of cells in culture. The mechanism of action was studied by determining the rate of apoptosis and expression of protein involved in signal transduction. The result indicated that the butanol extract of H. Odorata had selective inhibition to both Hep G2 and Chang cells with IC50 of 20.14 and 377 μg/ml, respectively. Growth inhibition by the extract showed an increased of apoptosis at concentration of 25 μg/ml. Cell study demonstrated morphologic changes characteristic of apoptosis such as chromatin condensation and fragmentation, as well as formation of apoptotic bodies. However, MAPK kinase signal transduction pathway indicated no difference in ERK1 and ERK2 expression level after exposure at varying time. P53 protein level also showed no changes in expression compared to control. In conclusion, the increase in apoptosis observed was not due to changes in MAPK pathways involving ERK1, ERK 2 and p53 but may involve other pathway, which require further investigation.
Source : Journal of Medicinal Plants Research Vol. 6(8), pp. 1382-1388, 29 February, 2012 Link to Full Article