Long-Term Treatment with Aqueous Garlic and/or Tomato Suspensions Decreases Ehrlich Ascites Tumors Jenifer Bom,1 Patrícia Gunutzmann,1 Elizabeth C. Pérez Hurtado,1 Jussara M. R. Maragno-Correa,2 Silvia Regina Kleeb,3 and Maria Anete Lallo1
1Environmental and Experimental Pathology Post-Graduation, Paulista University (UNIP), Rua José Maria Whitaker 290, 05622-001 São Paulo, SP, Brazil 2Pharmacology Department, Federal University of São Paulo (UNIFESP), Rua Sena Madureira, 04021-001 São Paulo, SP, Brazil 3Veterinary Medicine, University Metodista of São Paulo, Rua Alfeu Tavares 149, 09641-000 São Bernardo do Campo, SP, Brazil
Abstract We evaluated the preventive and therapeutic effects of aqueous suspensions of garlic, tomato, and garlic + tomato in the development of experimental Ehrlich tumors in mice. The aqueous suspensions (2%) were administered over a short term for 30 days before tumor inoculation and 12 days afterward, and suspensions at 6% were administered for 180 days before inoculation and for 12 days afterward. The volume, number, and characteristics of the tumor cells and AgNOR counts were determined to compare the different treatments. Aqueous 6% suspensions of garlic, tomato, and garlic + tomato given over the long term significantly reduced tumor growth but when given over the short term, they did not alter tumor growth.
It is well known that the garlic-derived organosulfur compounds (OSCs) are effective to inhibit a variety of human cancers such as prostate, breast, colon, skin, lung, and bladder cancers. Herein, the pro-apoptotic effects of diallyl trisulfide (DATS), one of garlic-derived OSCs, on T24 bladder cancer cells were investigated. The results demonstrated that DATS suppressed proliferation of T24 bladder cancer cells in a dose- and time-dependent manner which was associated with induced G2/M Phase cell cycle arrest and apoptosis. Mechanistically, DATS inhibits phosphatidylinositol 3'-kinase/Akt activation that, in turn, results in modulation of Bcl-2 family proteins, leading to enhanced apoptosis of T24 cells. These findings suggest that DATS may be an effective way for treating human bladder and other types of cancers.