Exogenous coenzyme Q10 modulates MMP-2 activity in MCF-7 cell line as a breast cancer cellular model Massih Bahar1 , Shahnaz Khaghani1 , Parvin Pasalar1 , Maliheh Paknejad1 , Mohammad Reza Khorramizadeh2 , Hossein Mirmiranpour2 and Siavash Gerayesh Nejad1
1 Department of Clinical Biochemistry, Tehran University of Medical Sciences, Faculty of Medicine, Tehran, Iran 2 Department of Pathology, Tehran University of Medical Sciences, Faculty of Public Health, Tehran, Iran
Background/Aims Matrix Metalloproteinases 2 is a key molecule in cellular invasion and metastasis. Mitochondrial ROS has been established as a mediator of MMP activity. Coenzyme Q10 contributes to intracellular ROS regulation. Coenzyme Q10 beneficial effects on cancer are still in controversy but there are indications of Coenzyme Q10 complementing effect on tamoxifen receiving breast cancer patients.
Methods In this study we aimed to investigate the correlation of the effects of co-incubation of coenzyme Q10 and N-acetyl-L-cysteine (NAC) on intracellular H2O2 content and Matrix Metalloproteinase 2 (MMP-2) activity in MCF-7 cell line.
Results and Discussion Our experiment was designed to assess the effect in a time and dose related manner. Gelatin zymography and Flowcytometric measurement of H2O2 by 2'7',-dichlorofluorescin-diacetate probe were employed. The results showed that both coenzyme Q10 and N-acetyl-L-cysteine reduce MMP-2 activity along with the pro-oxidant capacity of the MCF-7 cell in a dose proportionate manner.
Conclusions Collectively, the present study highlights the significance of Coenzyme Q10 effect on the cell invasion/metastasis effecter molecules.
Source :Nutrition Journal 2010, 9:62doi:10.1186/1475-2891-9-62 Link to Source
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