The Aqueous Soluble Polyphenolic Fraction of Psidium guajava Leaves Exhibits Potent Anti-Angiogenesis and Anti-Migration Actions on DU145 Cells Chiung-Chi Peng,1,2 Chiung-Huei Peng,3,4 Kuan-Chou Chen,5 Chiu-Lan Hsieh,6 and Robert Y. Peng4 1School of Physical Therapy, College of Health Care, China Medical University, 91, Hsueh-Shih Rd., Taichung, Taiwan 40202, Taiwan 2Graduate Institute of Rehabilitation Science, College of Health Care, China Medical University, 91, Hsueh-Shih Rd., Taichung, Taiwan 40202, Taiwan 3Department of Nursing, Hungkuang University, 34, Chung-Chi Rd., Shalu County, Taichung Hsien, Taiwan 43302, Taiwan 4Research Institute of Biotechnology, Hungkuang University, 34, Chung-Chi Rd., Shalu County, Taichung Hsien, Taiwan 43302, Taiwan 5Department of Urology, Taipei Medical University-Shuang Ho Hospital, Taipei Medical University, 250, Wu-Xin St., Xin-Yi District, Taipei, Taiwan 6Graduate Institute of Biotechnology, National Chang-Hua University of Education, 1, Jin-De Road, Changhua City 500, Taiwan
Abstract The aqueous extract of Psidium guajava budding leaves (PE) bears an extremely high content of polyphenolic and isoflavonoids. Whether it could be used as an anti-tumor chemopreventive in view of anti-angiogenesis and anti-migration, we performed the assay methods including the MTT assay to examine the cell viability; the ELISA assay to test the expressions of VEGF, IL-6 and IL-8; the western blot analysis to detect TIMP-2; the gelatinolytic zymography to follow the expression of MMPs; the wound scratch assay to examine the migration capability; and the chicken chorioallantoic membrane assay to detect the suppressive angiogenesis. Results indicated that the IC50 of PE for 5 cells was ~0.57 mg ml−1. In addition, PE effectively inhibited the expressions of VEGF, IL-6 and IL-8 cytokines, and MMP-2 and MMP-9, and simultaneously activated TIMP-2 and suppressed the cell migration and the angiogenesis. Conclusively, PE potentially possesses a strong anti-DU145 effect. Thus, clinically it owns the potential to be used as an effective adjuvant anti-cancer chemopreventive. .......We conclude that PE potentially possesses a strong anti-prostate cancer effect. Results suggest that PE can be used as an effective clinical adjuvant anti-prostate cancer chemopreventive.