Effects of Feiyanning Decoction on gene expression of nuclear factor-κB activated by tumor necrosis factor-α in lung adenocarcinoma cell line
1. Ju-yong WANG (Tumor Institute of Traditional Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China 2. Ye JIN (Department Bioengineering, School of Life Sciences, Shanghai University, Shanghai 200444, China ) 3. Zhen-ye XU (Tumor Institute of Traditional Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China ) 4. Zhan ZHENG (Tumor Institute of Traditional Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China )
Objective: To study the effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine, on gene expression of nuclear factor-κB (NF-κB) activated by tumor necrosis factor-α (TNF-α) in lung adenocarcinoma cell line (A549). Methods: A549 cells were incubated with rat serum containing Feiyanning at different concentrations for 24 and 48 h, respectively. Morphology of cells was observed by an inverted microscope after treatment with reagents. The cell proliferation was examined by 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt (WST-8) assay. The expressions of NF-κB and inhibitor κBα (IκBα) were studied by Western blotting. NF-κB-dependent luciferase reporter (3×κB-luc) was transfected for 24 h, and the cells were treated with the reagents for 24 h, and then the transcriptional activity of NF-κB promoter was detected by luciferase assay. Results: TNF-α (1 μg/L) strongly induced the expression of NF-κB by approximately 1.76-fold compared with the control in the nuclei of A549 cells, and the induced NF-κB expression was significantly suppressed by addition of Feiyanning (P<0.01). In addition, Feiyanning inhibited the transcriptional activity of the NF-κB promoter. However, we observed no significant changes in IκBα expression (P>0.05). Conclusion: Feiyanning Decoction can markedly inhibit human lung cancer A549 cell proliferation, which may be partly due to inhibition of NF-κB activation induced by TNF-α. It is therefore expected to be a new strategy for treating lung cancer.