Cytotoxicity Effects of Amoora rohituka and chittagonga on Breast and Pancreatic Cancer Cells Leo L. Chan,1 Sherine George,2 Irfan Ahmad,3 Saujanya L. Gosangari,4,5 Atiya Abbasi,6 Brian T. Cunningham,1,2 and Kenneth L. Watkin4,5,7
1Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA 2Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA 3Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA 4Bioimaging Science and Technology Group, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA 5College of Applied Health Science, University of Illinois at Urbana-Champaign, Champaign, IL 61820, USA 6Medical Imaging Research Laboratory, Department of Speech and Hearing Science, University of Jllinois at Urbana-Champaign, Urbana, IL 61801, USA 7International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan
Abstract Chemotherapeutic agents for cancer are highly toxic to healthy tissues and hence alternative medicine avenues are widely researched. Majority of the recent studies on alternative medicine suggested that Amoora rohituka possesses considerable antitumor and antibacterial properties. In this work, rohituka and chittagonga, fractionated with petroleum ether, dichloromethane, and ethanol, were explored for their anticancer potential against two breast cancer (MCF-7 and HTB-126) and three pancreatic cancer (Panc-1, Mia-Paca2, and Capan1). The human foreskin fibroblast, Hs68, was also included. Cytotoxicity of each extract was analyzed using the MTT assay and label-free photonic crystal biosensor assay. A concentration series of each extract was performed on the six cell lines. For MCF-7 cancer cells, the chittagonga (Pet-Ether and CH2Cl2) and rohituka (Pet-Ether) extracts induced cytotoxicity; the chittagonga (EtoAC) and rohituka (MeOH) extracts did not induce cytotoxicity. For HTB126, Panc-1, Mia-Paca2, and Capan-1 cancer cells, only the chittagonga CH2Cl2 extract showed a significant cytotoxic effect. The extracts were not cytotoxic to normal fibroblast Hs68 cells, which may be correlated to the specificity of Amoora extracts in targeting cancerous cells. Based on these results, further examination of the potential anticancer properties Amoora species and the identification of the active ingredients of these extracts is warranted.
Source : Evidence-Based Complementary and Alternative Medicine Volume 2011 (2011), Article ID 860605, 8 pages doi:10.1155/2011/860605 Link to Full Article